The congregation was established in eastern Toronto on July 12, 1842, for working-class families unable to pay the high pew prices at St. James', and so they built a church for all people.

During this renovation, the floor of the nave was raised 4 ft (1.2 m) to allow for construction of an activity hall on the lower level.

[4] Little Trinity has sent dozens of church members overseas to serve the development and spiritual needs of nations around the world.

In 2014, a new office space was opened at 403 King Street East, in a revitalized heritage building.

The present multi-generational congregation is made up of members of many backgrounds from across the Toronto region.