After the junction with the line to Stralsund, it turns to the northwest and passes through the Müritz National Park to Waren.

In Kargow there is the junction with the Waren (Müritz)–Neubrandenburg railway, which was dismantled after the Second World War for reparations.

Its goal was to a build railway on the shortest route from Berlin to Copenhagen from Neustrelitz, the location of the court of the Grand Duchy of Mecklenburg-Strelitz, via Rostock to the harbour of Warnemünde.

After two years of construction, the 113 km long section from Neustrelitz via Waren and Plaaz to Rostock was opened for freight on 1 June 1886.

On the same day the steamship line from Warnemünde to Gjedser (now Gedser) on the Danish island of Falster was put into operation.

The importance of the railway rose sharply on 1 October 1903 with the opening of the train ferry to Gedser.

From then on there were international express trains from Berlin to Copenhagen, which made the railway the most important link between the capital and Scandinavia.

On 29 December 1941, Wehrmacht train W 96 031 ran into two locomotives at the station Langhagen due to incorrectly set points.

[2] The line suffered the biggest set back in its history after the Second World War with the dismantling of the section from Neustrelitz via Waren and Lalendorf to Plaaz.

It proved necessary to reconstruct the line, partly because of the considerable freight traffic to the newly constructed port of Rostock.

Architectural evidence of the reconstruction are the characteristic single-storey station building in Scharstorf, Subzin-Liessow, Langhagen, Grabowhöfe, Klockow, Kratzeburg and Adamsdorf.

Electric services started on 19 May 1985, running initially via Güstrow and, from 15 December 1985, over the actual Lloyd Railway via Laage.

A bus service was established by Personenverkehr GmbH Müritz (PVM) from Waren via Kargow to Klockow.

Since the completion of the reconstruction between Rostock and Lalendorf in June 2007, an Intercity-Express service has operated six times a week from Warnemunde to Berlin and Munich.

The Berlin–Rostock is being upgraded as part of an investment program on the existing network for speeds of generally 160 km/h and higher axle loads of up to 25 tonnes.

[9] In June 2007, the first upgraded section between Rostock and Lalendorf Ost was reopened after a year of complete renovation.

[6] As part of the upgrade, the platform in Kratzeburg station is being extended and in future it will be served by the Rostock–Berlin Regional-Express services.

The traveling time of Regional-Express services between Neustrelitz and Rostock has not been reduced compared to the situation before the start of the work.