Lowlands Nature Reserve is located in the Swan Coastal Plain bioregion of Western Australia, on the Serpentine River.

[3] Lowlands Nature Reserve was gazetted on 2 December 2014 and has a size of 13.12 square kilometres (5.07 sq mi).

[1] The nature reserve is located in the west of the locality of Mardella, in the Shire of Serpentine-Jarrahdale.

It consists of three separate sections, with the two eastern ones separated by the narrow Lowlands Road corridor while the third section is approximately 1 kilometre (0.62 mi) further west, centered around Hymus Swamp.

[4] In 2017, a male western quoll was spotted at Lowlands, which is considered unusual given that the nature reserve is surrounded by agricultural land.