Lyne Shackelford Metcalfe (April 21, 1822 – January 31, 1906) was a U.S. Representative from Missouri.

He engaged in mercantile pursuits in Alton, Illinois, in 1844.

During the Civil War served in the Union Army as assistant quartermaster with rank of captain and later promoted to colonel.

He was an unsuccessful candidate for reelection in 1878 to the Forty-sixth Congress.

He died in Kirkwood, St. Louis County, Missouri, January 31, 1906.