The collection is dedicated to the geology, paleontology, flora and fauna of South Limburg.

Highlights from the collection are Cretaceous fossils from Sint-Pietersberg, most notably the skull of a Mosasaur (nicknamed "Bèr") and specimens of Hoffmann's giant turtle, Allopleuron hofmanni and Suyckerbuyk's turtle, Glyptochelone suyckerbuyki.

Multiple attempts have been made since 1824 to have the piece returned to Maastricht.

A remarkable piece from that collection is a rat king from the 19th century.

Behind the museum is a botanical garden, situated on the banks of the small river Jeker.