The Madonna of Humility is a tempera-on-panel painting by the Italian late medieval artist Gentile da Fabriano, dating from around 1420–1423 and housed in the Museo nazionale di San Matteo, Pisa.

The work, once in the local Pious House of Misericordy, was commissioned in unknown circumstances, although its size suggests that it was destined to private devotion.

It was perhaps ordered by cardinal Alemanno Adimari, archbishop of Pisa, who had his sepulchre in the Roman church of Santa Maria Nova decorated by Gentile (a lost work mentioned by late Renaissance art biographer Giorgio Vasari).

The Madonna of Humility, with the Virgin sitting on a cushion over the ground, was a common theme in early 15th century western painting.

The mantle of the Virgin has on the border the inscription "AVE M[A]T[ER] DIEGNA [D]EI", while on the edge of the cloth on which the Child lies are Arabic characters, which have been read as the Quran Shahada (لا إله إلا الله - Lā ilāha illa Allāh).