The stream's course dictates the unusual placing of the entrance which is on the north wall instead of the normal, west of centre.

[7] It is a classic site for the nationally scarce Cornish moneywort (Sibthorpia europaea), first reported here in 1824.

[8][9] The Penzance Natural History and Antiquarian Society visited the well and chapel in August 1888 and the members were shown two insectiferous plants; pale butterwort (Pinguicula lusitanica) and round-leaved sundew (Drosera rotundifolia).

[10] The nearby Madron Well, which is now concealed in shrubs and undergrowth, is an example of a Cornish Celtic sacred site and is a ground level natural spring.

[6] The well is said to have healing properties and a 17th-century written account tells how, before 1641, John Trelille, a poor cripple, was cured here when he bathed in the water, then slept on a grassy hillock.

[6] An old May Day tradition, which was still being observed in 1879, was for many young folks (mainly girls) to head from Penzance before sunrise, to perform a ceremony, to learn the number of years they have to wait before they get married.