The section between Alzey and Armsheim is also used by services on the Rheinhessen Railway from Bingen to Worms.

As the line from Armsheim to Mainz is single-track only, services can be operated at most every half-hour, with oncoming trains forced to wait in stations, usually in Saulheim and Mainz-Marienborn (RB) or Mainz-Gonsenheim (RE).

[3][4] Until the timetable change in December 2014, the line was operated by DB Regio, after which vlexx GmbH took over the service from Mainz to Kirchheimbolanden.

The Elsass-Express (Alsace Express) to Wissembourg uses this part of the line on weekends and on public holidays.

vlexx GmbH has operated Alstom Coradia LINT diesel multiple unit since December 2014.