Marsa and St. George's were the teams which were relegated from the 2006–07 Maltese Premier League.
Dingli Swallows and Mellieha were the promoted teams from the 2006–07 Maltese Second Division.
Tarxien Rainbows finished as champions and were promoted to the 2008–09 Maltese Premier League.
They were joined with Qormi, who won a play-off with Mosta 4-3 on penalties, following a 0-0 draw.
The play-off was necessary because the two teams finished level on points.