It runs 41.6 kilometres (25.8 mi) from the Donaustadt district of Vienna to Marchegg, where it crosses the Morava river and enters Slovakia.

The line splits from the Laaer Ostbahn near Wien Stadlau railway station, in the Donaustadt district of Vienna.

From Marchegg, the line crosses the Morava river and enters Slovakia.

[2] Electrification was extended to Marchegg in December 2022; work on double-tracking continues.

[3] Works to electrify the section from Marchegg to Devínska Nová Ves are going to start in March 2024 with the opening scheduled for December 2024.