He was born into a reasonably affluent noble family in Forlimpopoli, Forlì, who had owned land in the area since the early 14th century.
Many members of the family held ecclesiastical posts locally, including Uccellini's father Pietro Maria, and it is likely that Marco went to study at the seminary in Assisi sometime in the early 1630s.
According to payroll records and remnants of Uccellini's correspondence, it is clear that he was a valued advisor and confidant to the d'Este family, paid almost eight times more than the other court violinists.
[3] Some examples are: He also composed one opera, Gli eventi di Filandro ad Edessa, which premiered in Parma in 1675.
Besides introducing several technical innovations necessary to play his difficult music, he was an early popularizer of music written explicitly for solo violin and continuo; at the time, it was common for composers not to specify instruments in their works, preferring to write parts adaptable between instruments of similar ranges.