She was the daughter of the previous earl (and also Duke) of Rothes, John Leslie, who was the 7th Earl and 1st Duke of Rothes.

On 8 October 1674, she married her cousin Charles Hamilton, 5th Earl of Haddington, making her children thus Hamilton's.

Under the terms of her father's earldom, Hamilton took the surname of Leslie, and arranged to pass his own peerage to the second son.

[1] She had four children, the eldest of whom was John Hamilton-Leslie who became Earl after her death in August 1700.

[2] On 8 October 1674 she married her cousin Charles Hamilton, 5th Earl of Haddington.