Marie Podvalová (5 September 1909 – 16 May 1992) was a Czech opera singer who had a long career at the National Theatre in Prague from 1936 to 1978.

A dramatic soprano who excelled in the Czech repertoire, she garnered particular acclaim for her portrayal of the title heroine in Bedřich Smetana's Libuše.

She made her professional opera debut in 1935 at the Mahen Theatre in Brno as Marina in Modest Mussorgsky's Boris Godunov.

[1] Under the recommendation of conductor Václav Talich, Podvalová was made a principal artist at the Prague National Theater in 1936.

Among her signature roles at the opera house were Emilia Marty in Leoš Janáček's The Makropulos Affair, the Foreign Princess in Antonín Dvořák's Rusalka, Kostelnička Buryjovka in Janáček's Jenůfa, Leonora in Fidelio, Milada in Smetana's Dalibor, Senta in Richard Wagner's The Flying Dutchman, and the title roles in Giacomo Puccini's Tosca and Smetana's Libuše.