Marienborn is a village and a former municipality in the Börde district in Saxony-Anhalt, Germany.

In 1191 Wichmann von Seeburg, then Archbishop of Magdeburg, established an asylum at the site of an apparition of the Virgin Mary and a spring (Born) with healing waters, which during the 13th century evolved into a monastery for Augustinian nuns.

The sisters of Marienborn abbey left a monastery church and cloister with foundations from about 1200, while the pilgrimage chapel at the spring is a replica dating from the 19th century.

Marienborn has access to the Bundesautobahn 2 and the Bundesstraße 1 federal highway.

The former railway border crossing at Marienborn station today is a stop on the railway line from Braunschweig to Magdeburg, served by Regionalbahn trains of the Deutsche Bahn company.