Mario Strikers Charged supported the Nintendo Wi-Fi Connection, which permitted players to participate in online matches and tournaments.

A new element for the game is the powerful shot possible is the "Mega Strike", which only the captain can perform and accounts for a varying number of points ranging from three to six.

In addition, sidekicks are able to perform a special shot—known as a "Skillshot"—that occurs if sufficiently charged and results in the character releasing a shot that is either able to paralyze or bypass the goalkeeper.

Some power-ups aid the player by immobilising and hindering the opponent, while others grant temporary invulnerability and allow the user to score a goal more easily.

The player can also play through "Striker Challenges", where they select a session with certain obstructions, such as missing characters or a susceptible goalkeeper.

If they are successful in passing, they are given further adjustments called "cheats", that can render shooting easier and turn off physical elements of the fields in the game.

Training sessions appear in the form of "Strikers 101," where the player can practice individual aspects such as shooting and tackling.

Unlike other Mario sports games, players must restart the cup from the beginning when they lose in the elimination round, rather than being given the option to replay the match that they lost in.

A lot of challenges require the player to win such as the goalie not being able to catch the ball because of being weak to charged shots, or the opposing team leading by 5 points by the start of the match.

[24] Hyper's Rico Shavez commended the game for its "great net code" and being "immediately fun".