He was a pupil of Cosimo Rosselli, in whose workshop he met Baccio della Porta, later known as Fra Bartolomeo.

[1][3] Albertinelli's earliest works include a small triptych of the Madonna and Child with Saints Catherine and Barbara (1500) at the Museo Poldi Pezzoli, Milan, and another triptych of the Madonna and Child with Saints, Angels and Various Religious Scenes at the Musée des Beaux-Arts in Chartres.

The several panels with Scenes from Genesis, at the Courtauld Institute in London, Strossmayer Gallery in Zagreb, Accademia Carrara in Bergamo and Harvard Art Museums in Cambridge, probably also date from this period.

[1] In 1503 Albertinelli signed and dated his best-known work, an altarpiece for the chapel of Sant'Elisabetta della congrega dei Preti in San Michele alle Trombe, Florence (now in the Uffizi).

[1] The pyramidal composition, classical background architecture and pronounced contrasts of light and dark make the painting a quintessential example of High Renaissance art.