[2] She is related to several notable artists from the Kwakwaka'wakw art tradition, including Ellen Neel, Mungo Martin, and Charlie James.
[4] Barkhouse began her professional career in the 1990s[4] and has since created works of art that explore contemporary environmental and indigenous concerns, using personal and collective histories and often incorporating animal imagery.
[3] One of Barkhouse's most significant works is Harvest, a mixed media sculpture completed in 2009 for the Muhheakantuck in Focus exhibition at Wave Hill in the Bronx, NY.
[8] Installed at the McMichael Gallery in Vaughan, Ontario in 1998, it includes several bronze sculptures of wolves, and a transit shelter with a poster of a raven.
[10] In 2013, The Canadian Museum of History installed 'namaxsala (To Travel in a Boat Together), a bronze and copper sculpture of a wolf in a canoe, staring across the Ottawa River at Parliament Hill.
[4] In 2017, the Koffler Centre of the Arts in Toronto organized a major solo exhibition of new and past works, Mary Anne Barkhouse: Le rêve aux loups, curated by Jennifer Rudder.