Mary Florence Denton (July 4, 1857 – December 24, 1947) was an American educator in Japan, and a longtime member of the faculty at Doshisha University in Kyoto.

She went to Japan in 1888, supported by the American Board of Commissioners for Foreign Missions, and taught English, Bible study, and cookery classes for many years at the Doshisha University in Kyoto.

[2][3] She raised funds for the school from American benefactors, lectured on Japan during her few furlough visits to the United States,[4][5][6] and encouraged Doshisha students, including Hisa Nagano and Taki Handa, to pursue further study abroad.

[11] In 1936, she hosted a visiting American art collector, Lilla Cabot Perry, who wrote about Denton, "She is doubtless in her late eighties, but made of wire and steel.

Because of her advanced age and frailty, and out of respect for her long service to the school, Doshisha College arranged for her to stay in her home, officially on house arrest, for the war's duration.