Matheus Biteco was born in Porto Alegre, Rio Grande do Sul, and joined Grêmio's youth setup in 2001 at the age of five.
[2] He made his senior debut on 1 February 2013, coming on as a second half substitute in a 0–4 Campeonato Gaúcho away loss against São Luiz.
In December 2014, Matheus Biteco's federative rights were sold to a group of businessmen, being assigned to Santa Catarina-based club Barra and being loaned back to Grêmio for the season.
[5] At Chape Matheus Biteco became a regular starter, also helping the side to reach the 2016 Copa Sudamericana Finals for the first time in their history.
On 28 November 2016, whilst travelling with Chapecoense to the aforementioned finals, Matheus Biteco was among the fatalities of the LaMia Flight 2933 accident in the Colombian village of Cerro Gordo, La Unión, Antioquia.