He studied medicine in Paris, and later served as prosector in Strassburg.
In 1873 he became agrégé, subsequently becoming director of the anthropological laboratory at the École des Hautes Etudes and an anatomy professor at the École Supérieur des Beaux-Arts.
In 1885 he replaced Charles-Philippe Robin (1821–1895) as professor of histology at the medical faculty in Paris.
[1] Duval is remembered for research involving placental development in mice and rats, and was the first to identify trophoblast invasion in rodents.
With Austrian-American gynecologist Walter Schiller (1887–1960), Schiller Duval bodies are named, which are structures found in endodermal sinus tumors.