In Singapore, she founded the Convent of the Holy Infant Jesus girls' school and later traveled to Japan where she established a hospice and homeless shelter.
The sisters landed in Penang in October 1852 and began work at an orphanage and school before traveling to Singapore in February 1854 under request of Father Jean-Marie Beurel.
Mother Mathilde remained in Singapore and eventually establishing Christian girls' school the Convent of the Holy Infant Jesus and opening an orphanage to care for abandoned children.
In 1872, Mother Mathilde and her Sisters traveled to Japan where they founded the Saint Maur School at Yokohama, Kanagawa Prefecture to teach and care for Japanese children.
On 20 January 1911, Mother Mathilde Raclot died at the age of 96 and was buried at the Yokohama Foreign General Cemetery.