Matthias Mann (born 10 October 1959) is a German physicist and biochemist.

Born in Thuine, Lower Saxony, he studied mathematics and physics at the University of Göttingen.

He received his Ph.D. in 1988 at Yale University where he worked in the group of John Fenn, who was later awarded the Nobel Prize in Chemistry.

After a postdoctoral fellowship at the University of Southern Denmark in Odense, Mann became group leader at the European Molecular Biology Laboratory (EMBL) in Heidelberg.

In addition, he became a principal investigator at the Novo Nordisk Foundation Center for Protein Research in Copenhagen.