[1] It is prepared by cyclization of cis-N-methyl-3-thiocyanoacrylamide:[2] The crystal and molecular structure of methylisothiazolinone, as determined by in situ cryocrystallography, was reported for the first time in 2024.

In addition, this product serves as an antimicrobial agent in latex adhesives and in paper coatings that also contact food.

[11] Additionally, new research into cross reactivity of MIT-sensitized patients to variants benzisothiazolinone and octylisothiazolinone have found that reactions may occur if present in sufficient amounts.

[12] In the United States, the Environmental Protection Agency has a 1998 data sheet on methylisothiazolinone in their Pesticides section [13] which reads in part "Human Health Assessment: Toxicity: In studies using laboratory animals, methylisothiazolinone has been shown to be of moderate acute toxicity by the oral and inhalation routes.

In 2014, the European Commission Scientific Committee on Consumer Safety further issued a voluntary ban on the MCI/MI mixture from leave-on products such as body creams.