Mikulovice (German: Niklowitz) is a market town in Znojmo District in the South Moravian Region of the Czech Republic.

The town is situated on the right bank of the Jevišovka River.

Horní Dunajovice Reservoir, built on the Křepička Stream, is situated on the northeastern municipal border.

In 1558, Mikulovice was promoted to a market town by Emperor Ferdinand I.

The main landmark of Mikulovice is the Church of Saints Peter and Paul.