Other notable roles include that of Chun-Li in Street Fighter (1994), Detective Ellen Yin in The Batman (2004–2005), and Camile Wray in Stargate Universe (2009–2011).

Wen's parents divorced when she was an infant, and she moved with her brother and mother to Hong Kong, where she attended a Catholic school.

Her mother remarried to Chinese American Soo Lim Yee, and when Wen was four years old, the family moved to New York City.

After five years, Wen's mother and stepfather relocated their family to the Pittsburgh, Pennsylvania area, where they bought and operated the Chinatown Inn restaurant (established in 1943), which is still in business.

[citation needed] After starring in the film The Joy Luck Club (1993),[9] Wen landed the role of Dr. Jing-Mei "Deb" Chen on the NBC drama series ER.

Her next TV role was an FBI agent in the Fox kidnapping drama series Vanished, which premiered in the fall of 2006, but it too was cancelled.

From December 5 to 6, 2008, Wen starred in a benefit production of the musical Grease with "Stuttering John" Melendez at the Class Act Theatre.

[12] Wen was cast as a regular in the Stargate Universe television series from October 2009 to May 2011 as political attaché Camile Wray.

[15] In August 2014, Wen reprised her role as Mulan for the first time since Kingdom Hearts II in the Disney Channel series Sofia the First.

[18] In 2019, it was announced that Wen would be a cast member of the Star Wars TV series The Mandalorian playing Fennec Shand.

[19] She reprised the role vocally in Star Wars: The Bad Batch[20] and the live-action series The Book of Boba Fett.

[19] In 2022, Wen made an appearance on Young Sheldon as Dr. Lee, an experimental physicist responsible for mitigating conflict within a team of scientists.

[28] WDW Magazine writer Aaron Widmar called Wen an "incredibly talented Chinese actress, who has had a remarkable career that seems to only gain steam as it progresses — a rarity in Hollywood".