Former MS 16 heads south enter town along Martin Luther King Jr. Street before turning left onto Rosenwald Avenue just north of downtown.

They headed south along a three-lane boulevard through a business district before state maintenance restarts on MS 16, with it splitting off along McLaurin Street.

MS 16 leaves Rolling Fork as a two-lane and passes southeast through farmland for a few miles to enter the Delta National Forest.

It travels southeastward through the northern part of for several miles before leaving the forest and crossing the Sunflower River into Holly Bluff and Yazoo County.

MS 16 travels southeast across the Mississippi Band of Choctaw Indians Reservation, where it passes through the community of Pearl River, as well as widens to a four-lane divided highway for several miles.

The highway passes through a business district before having a short concurrency with MS 39 as it bypasses downtown along its north side.

MS 16 leaves De Kalb and travels through more remote woodlands (where it crosses the Sucarnoochee River) to enter Scooba.

The Martin Luther King Jr. Street and Rosenwald Avenue portions were turned over to the city of Rolling Fork, with the former concurrency with US 61/MS 14 being annulled at that time.