MS 316 begins at the eastern city limits of Friars Point along Rozell Lane.

The road continues east through agricultural fields until it skits the southern limits of Coahoma and has a grade crossing with the Mississippi Delta Railroad.

In the town, MS 316 travels along Coahoma Road and Main Street.

Past the town's eastern limit, state maintenance begins and the MS 316 is signed.

The road parallels Moore Bayou towards the southeast before heading east into Quitman County.