The route started at MS 8 near Gore Springs, and it traveled northward over eastern Grenada County.

MS 335 then crossed over the Grenada Lake and entered Yalobusha County.

The route continued northward and ended at MS 330 near Gums.

It was extended southwards in 1960 to MS 8, before being removed from the state highway system by 1967.

[3][1] The route entered Yalobusha County north of Hendrix Road.