No towns are on the route, but the Mark Twain Birthplace State Historic Site is less than a mile to the east in Florida.

The road crosses a part of Mark Twain Lake before leaving the state park and reaching an intersection with the eastern terminus of Route U.

Route 107 continues northwest and crosses another portion of Mark Twain Lake prior to curving north again and passing through more woodland.

The road heads to the northwest and runs through a mix of farm fields and wooded areas.

Route 107 curves north and continues through agricultural areas before ending at US 24 southwest of Monroe City.