Its eastern terminus is at U.S. Route 67 about six miles (10 km) east of De Soto; its western terminus is at Route 21 a few miles north of De Soto.

De Soto and Olympian Village are the only cities on the route.

The route passes through wooded areas with some fields and homes, crossing over Union Pacific's De Soto Subdivision and coming to an intersection with Route P. The road leaves the De Soto area and heads east through more rural areas with some development.

Farther east, Route 110 comes to its eastern terminus at an interchange with US 67 in Olympian Village, where Route CC heads to the north.

The CKC Route 110 follows I-35 from Claycomo northeast of Kansas City to US 36 in Cameron then follows US 36 (and the short part of I-72) to Hannibal.