Route 163 passes through Rock Bridge State Park and Columbia is the only city on the highway.

Route 163 originally began at an at-grade intersection before being removed when a new US 63 southbound lane was built.

After two miles (3.2 km) running parallel to US 63, Route 163 turns west and then passes in a northwesterly direction through Rock Bridge State Park.

North of the school, the highway intersects Route AC, which is known locally as Nifong Boulevard.

After crossing Hinkson Creek, Route 163 passes near the University of Missouri sports complex, including Faurot Field and the Mizzou Arena.