Missulena manningensis is a species of mouse spider in the Actinopodidae family.

It was described in 2021, with the specific epithet manningensis referring to the type locality.

[1][2] The species is found in the Murchison IBRA bioregion of Western Australia.

[2] It is known only from the Mount Manning area.

The holotype specimen was obtained in tall open eucalypt woodland with mixed shrubs.