Nasir began his career as a professor at the Diponegoro University, where he was also dean of the Masters of Accounting Program in the Faculty of Economics.

In early 2014 after serving as dean for four years, he was appointed rector of Diponegoro University and was said to sworn in officially on 18 December 2014.

[5] On 27 October 2014, he was appointed minister by President Joko Widodo several weeks before his official swearing in as rector of Diponegoro University.

[6][7] Nasir Muhammad at the beginning of 2016 had issued an idiotic, hateful, and controversial statement against the lesbian, gay, bisexual, and transgender (LGBT).

Later on 26 January 2016, the minister of education, Nasir stated that LGBT individuals are allowed to be students of Indonesian campuses.