[1] There is interest in using M. neglectum for the production of biofuels because it can accumulate large quantities (up to 33% of cell dry weight) of triacylglycerides (TAGs).
[4] The mitochondrial genome of M. neglectum is 96 kilobytes, which is twice as large as Nannochloropsis gaditana and nearly six times larger than the green alga Chlamydomonas reinhardtii, but contains fewer genes.
[4][5] A nuclear transformation technique has been established for M. neglectum using electrotransformation as the method of DNA delivery.
[7] In total, the transcriptome was sequenced at 12 time points (t0, 7x -N, 4x NR), yielding a comprehensive and high-quality dataset.
[7] It is furthermore possible to compare the expression patterns of target genes of M. neglectum with those of other microalgae which were also subjected to -N conditions to induce TAG accumulation.