They ascend into the white matter of the cerebellum, where each axon branches to innervate granule cells in several cerebellar folia.
In this case, the pathway is so named for a unique synapse formed by its projections, the mossy fiber rosette.
Fine branches of the mossy fiber axons twist through the granule cell layer, and slight enlargements giving a knotted appearance indicate synaptic contacts.
These contacts have the appearance of a classic Gray's type 1 synapse, indicating they are glutamatergic and excitatory.
Extensive branching in white matter and synapses to granular cells ensures that input from a single mossy fiber axon will influence processing in a very large number of Purkinje cells.