The station has separate entrances to each platform; these are connected by a footbridge which also serves as a public right of way.

The entrance to Platform 1 (the westbound platform) is via a set of steps from Queensdown School Road, a steeply sloping cul-de-sac off the main Lewes Road.

Platform 2 is reached from a path leading from the end of Crespin Way, another cul-de-sac at the eastern edge of the Hollingdean estate.

All services at Moulsecoomb are operated by Southern using Class 377 EMUs.

The typical off-peak service in trains per hour is:[2] During the peak hours, a number of additional services between Brighton, Hastings and Ore also call at the station.