Mount Barker Junction railway station is a disused station on the Adelaide to Wolseley line serving the South Australian city of Mount Barker.

[1] Mount Barker Junction station opened in 1883, and consisted of a main platform with a timber station building identical to the one at Balhannah, with a tall signal box next to the building.

It became a junction station when the Victor Harbor line opened.

SteamRanger subsequently relocated from Dry Creek to Mount Barker, and runs broad gauge services south on the line from Mount Barker station.

[2] Until 2005, SteamRanger ran a Junction Jogger service from Mount Barker station to this location, but ceased probably due to a worn-out track along this section.