Both men's and women's teams exist for basketball, cross country, golf, lacrosse, soccer, tennis, and track and field.

In the 2024-25 academic year, the Mules intend to add wrestling as an eleventh women's sport at the college.

The basketball, wrestling, and volleyball teams hold their games and matches at Memorial Hall, an indoor arena located on the campus at 2346 Liberty Street.

[5] In his first season, he guided the Mules to a 9–1 record[6] and a national championship with Muhlenberg defeating St. Bonaventure University in the Tobacco Bowl.

In 2007, the Mules again won the Centennial Conference and received another berth in the NCAA playoffs, winning their first-round game before falling in the second round.

Since the 2000 season, Muhlenberg has compiled a 66–28 overall record in the Centennial Conference, second best among all active and former members behind only Johns Hopkins University.