The original building at 308 Washington Avenue was listed on the National Register of Historic Places on October 7, 1994, but was delisted on May 12, 2009.

The 2004 Mukwonago Indians football team had an undefeated[4] season ending with a victory over the Marshfield Tigers in the state championship.

[5] Other team state championships include girls' swimming (2011, 2012, 2013), girls' cross country (1994), boys' cross country (1964, 1966),[6] boys' track (1964), as well as titles in debate and dance team.

MHS formerly had a competitive show choir, the women's-only "Starstruck", as well as the noncompetitive mixed-gender "Guys and Dolls".

Started in 2022, the competition features teams from across the Midwest coming to compete on the field and for judged awards.