A former student of the Escuela Normal Numero 6 de Palermo, Santa Ana attended high school with singer Érica García and actress Marcela Guerty.

At age 18 she began studying theater with Agustín Alezzo and then continued with other teachers such as Juan Carlos Gené, Rubén Szuchmacher, and Guillermo Angelelli.

In that program she met Mike Amigorena, who in 2006 invited her to join an experimental rock-pop theatrical music band, Ambulance, along with other actors such as Mariano Torre, Luciano Bonanno, Julián Vilar and Víctor Malagrino to later transform into an Orchestra-show by Sergio D'Angelo.

That year she also participated in the telenovela Lalola playing the character of Graciela, which earned her public recognition and for which her obtained her first nomination for Martín Fierro Awards in 2007.

In 2009 she was summoned by the director Juan Taratuto to be the protagonist of the telenovela Ciega a citas, based on a blog by Carolina Aguirre, with the character of Lucía González, a journalist who will have 258 days, until the date on which her sister will marry to find a boyfriend to suit her and thus be able to take revenge on her mother.