M. uniraptor is a pupal parasitoid of synanthropic filth-breeding Diptera and is a natural enemy of the housefly Musca domestica and the stable fly Stomoxys calcitrans.

It can generally be found in the reproductive organs of its host species, and can be transferred from female to offspring through the egg cytoplasm.

The actual process of gamete duplication differs among the insects that have been infected with Wolbachia, including a similar species of wasp called Muscidifurax raptorellus.

The bacterium has been known to cause negative side effects such as cytoplasmic incompatibility, thelytoky, and feminization in the reproductive habits of these various insect species.

[3] However, the bacterium, Wolbachia, has evolved with this species of wasp through vertical transfer to induce irreversible thelytokous reproduction of only female offspring.