It has an extensive sculptural collection ranging from the Middle Ages to the 19th century.

The collections come mostly from churches and monasteries in the Region of Castile, whose pieces of religious art were confiscated by the State in 1836, by order of Minister of Finance Mendizábal.

The museum houses works from the 13th to 19th centuries, executed mostly in the Central Spain, and also in other regions historically connected to Spain (Italy, Flanders, Southern America).

Artworks include, among the others, a Raising of the Cross by Francisco del Rincon, I Thirst, and The Way of Calvary Gregorio Fernández, Adoration of the Magi by Alonso Berruguete, Lamentation of Christ by Juan de Juni, Penitent Magdalene by Pedro de Mena or the Holy Sepulchre or passage of the Sleepers Alonso de Rozas.

During the Holy Week in Valladolid the museum gives 104 images (distributed in the corresponding pasos) to the processions for the brotherhoods.