Its collections include many artifacts from the museum of religious art of the Catholic Archbishopric of Utrecht, located in the convent until 1979.

The museum has an extensive collection of historical and art-historical objects from the early Middle Ages to the present.

The vast collection presents a picture of Protestant and Catholic art and cultural history of the Netherlands, and its impact on Dutch society.

The collection includes richly illustrated manuscripts, book-bindings decorated with precious stones, richly-worked images, paintings, altarpieces, pieces of clothing and ecclesiastical objects in gold and silver.

Dutch painting of the 16th and 17th centuries is represented by a selection of works by Jan van Scorel and Pieter Saenredam, and more recent Dutch artworks by Jan Toorop, Shinkichi Tajiri, Frans Franciscus, Guido van der Werve and Marc Mulders [nl].