The Board of Public Instruction granted the future museum the use of a converted Quonset style hut located at the Mary Karl Vocational School, on what is now the Campus of Daytona State College.

This necessitated a move from the original Quonset style building, and in 1964 the museum relocated to a new facility located on Daytona’s Tuscawilla Preserve.

The L. Gale Lemerand Wing was originally built on a dip in the property and flooded in May 2009 due to heavy rains.

In 2014 the Planetarium was rebuilt as a new state-of-the-art facility, incorporating a fully digital projection system as well as an exhibit space.

[5] The Charles and Linda William's Children's Museum features hands-on science exhibits in a 9,000-square-foot (840 m2) state-of-the-art facility.

), Indy race cars sponsored by the family, and one of the largest known collections of historic Coca-cola artifacts and memorabilia in the world (2,490 objects featuring original molds and the original patents for the bottle),[6] Apart, from the Museum's main campus, MOAS is steward to Gamble Place; A historic district nestled among the Spruce Creek Preserve.

Gamble Place has been developed and restored by the Museum of Arts & Sciences in cooperation with the Nature Conservancy and the City of Port Orange.

The preserve protects virgin Florida coastal hydric hammock, and is a habitat for endangered species of flora and fauna.