After killing an entire shopping district out of misunderstanding and grief-fueled rage, Rin meets Rei (Yuko Takayama), another mutant.

Rei introduces Rin to a small rag-tag group of mutant rebels led by a transvestite samurai named Kisaragi (Tak Sakaguchi), who is bent on restoring the Hiruko clan.

Until three years ago, Rei had been a freak at a circus, with the only person to show her compassion and acceptance being a boy who gave her a gold cross necklace.

When Rin achieves control over her powers much sooner than Kisaragi had expected, he orders her, along with Yoshie, to infiltrate an anti-Hiruko conference and attack a high-ranking general named Koshimizu.

Rin returns Rei's treasured gold cross necklace and triggers fond memories of the human boy who loved her.

[4] At the Sitges Film Festival, Mutant Girls Squad won the award for Best Motion Picture in the Midnight X-Treme section.

"[10] Variety gave the film a positive review, praising the directors as filmmakers who "execute a virtually nonstop exhibition of over-the-top violence with considerable creativity and visual flair.".

The review went on to praise screenwriter Jun Tsugita who included a "wealth of zingy lines" and the actors "Sugimoto, Takayama and Morita credibly negotiate the dialogue and show plenty of zest while lopping the limbs off all comers"[11] A review in The Montreal Gazette said he could not "assign a rating to Mutant Girls Squad...if you like this sort of extreme, gonzo gore then you'll be pleased with the carnival of tongue-in-cheek, perverted horrors on display here.