Tests are made on both, protein and DNA level, MutationTaster is hence not limited to substitutions of single amino acids but can also handle synonymous or intronic variants.
Mutation Taster is written in Perl and can process Next-generation sequencing data of all major platforms (Roche 454, Illumina Genome Analyzer and ABI SOLiD).
These tests comprise (among others): Integrated data sources (among others): The single results are then assessed by a Naive Bayes classifier which decides whether or not their combined effect might be deleterious for the protein.
The 'raw' accuracy of MutationTaster is about 90%, with the inclusion of knowledge about common (harmless) polymorphisms and known disease mutations, the actual rate of correct classifications is much higher.
MutationTaster is hosted at the Charité Berlin and its current developers are Olivia Ebner, Daniela Hombach, Markus Schülke, Jana Marie Schwarz, Dominik Seelow.