[3] It is difficult to grow in laboratories due to its complex nutritional requirements and the high chances of contamination associated with mycoplasma culture.

To successfully grow the bacterium, an environment of 5–10% carbon dioxide is required, and the medium should demonstrate an acid colour shift.

[citation needed] This mycoplasma is not known to produce any specifically harmful toxin like many other disease-causing bacteria, but some mildly toxic by-products have been observed.

[7] Mycoplasma hyopneumoniae has been a topic of interest in the scientific community due to the economic impact of porcine enzootic pneumonia.

[citation needed] Research has been mainly focused on identifying adhesins with a final goal of developing an effective vaccine that prevents M. hyopneumoniae from attaching to lung cilia.