According to the "Investment Atlas of Ukraine", Mykolaiv's railway station receives an average of 1.2 million passengers every year.

The largest destinations include: Kyiv, Odesa, Kharkiv, Dnipro, and Lviv, Zaporizhzhia, Kryvyi Rih, Kherson, and Ivano-Frankivsk.

On July 5, 2016, a new railway track running between Kyiv and Kherson opened, which includes a stop in Mykolaiv.

[2] On November 14, 2022, the first train left Mykolaiv station since the Russian invasion of Ukraine, and headed for its destination of Kyiv.

[3] There are also trains that serve secondary cities, such as to Mykolaiv (Lviv Oblast), Dolynska, Kakhovka, Tokarivka, and Apostolove.