NY 395 begins at an intersection with NY 7 (Duanesburg Road), Darby Hill Road (unsigned County Route 133 or CR 133) and Schoharie Turnpike (CR 74) in the hamlet of Quaker Street in the town of Duanesburg.

[3] NY 395 continues northeast through the village of Delanson, reaching a junction with Cole Road (CR 84).

At this junction, NY 395 turns northward once again, paralleling the railroad line back into Duanesburg.

After a bend to the northwest, the route passes the Delanson Reservoir and reaches an intersection with US 20 (Western Turnpike).

The right-of-way and the designation of NY 395 both terminate at the intersection, east of the Schoharie County line.