Nannie de Villiers (born Esmé de Villiers, 5 January 1976) is a former professional tennis player who represented South Africa.

She was born in neighbouring Namibia but moved at a young age.

De Villiers made her début in 1993, at the small ITF Johannesburg tournament.

Although she officially retired in 2003, she made a minor-comeback in 2007, entering the Cape Town event, losing in the first round singles and reaching the semifinals doubles.

She never surpassed the singles qualifying stages at a Grand Slam tournament.